MB (ASCP) EXAM 2023-2024 ATUAL EXAM 300
QUESTIONS AND CORRECT DETAILED
ANSWERS (100% VERIFIED ANSWERS)
|ALREADY GRADED A+
Consider the probe sequence, CTACCGTAATATTCGACCGT, to be
used in a hybridization procedure. What is the melting temperature, Tm,
of the sequence?
60°C
58°C
64°C
62°C – ANSWER- 58°C
This polymerase acts on DNA and produces Ribosomal RNA:
RNA Pol I
DNA Pol I
RNA Pol II
DNA Pol II – ANSWER- RNA Pol I
Sickle cell disease is an autosomal genetic disease due to a point
mutation in the beta-globin gene, where glutamic acid is substituted for
valine at the sixth codon of the gene, resulting in a faulty hemoglobin S
(Hb S). Sickle cell disease is one of many genetic diseases where a
single gene controls the expression of many phenotypic traits. The
phenomenon where a single gene controls the expression of many
phenotypic traits is best referred to as: – ANSWER- Pleiotrophy
What assay amplifies the target using a combination of a three-enzyme
system?
Branched DNA
TMA
PCR
NASBA
LCR – ANSWER- NASBA
In what part of a qPCR Amplification Plot do you take your
measurement? – ANSWER- Exponential phase
All of the following are liquid tumors, except:
Mantle cell lymphoma
Ewing sarcoma
Burkitt’s lymphoma
Acute promyelocytic leukemia – ANSWER- Ewing sarcoma
A technologist uses the spectrophotometer to quantify the amount of
DNA extracted from a blood specimen diluted 1:30. The absorbance
reading at 260 nm was found to be 2.545. If the absorbance at 280 nm
gave a reading of 1.406, and if the the DNA extract was re-suspended in
0.800 mL of EDTA solution, the DNA yield is: – ANSWER- (2.545 *
50ng/mcl)= 127.25
(127.25ng/mcl * 0.30) = 38.175ng/mcl
38.175ng/mcl ( 0.800mL1000) =
3054 micrograms
According to the wobble hypothesis, a U in the 5′ position of the anitcodon can pair with:
U or C
A or G
A, C or G
Only A – ANSWER- A or G
This polymerase acts on DNA and produces Messenger RNA: –
ANSWER- RNA Pol II
What assay amplifies the probe signal and the probe RNA concentration
increases if the target to be detected is present?
Branched DNA
TMA
PCR
NASBA
LCR
Qβ replicase – ANSWER- Qβ replicase
This restriction enzyme “digests and adds a methyl group from
Adenine”:
Type I
Type II
Type III
DNase I – ANSWER- Type III
What increases the half-life of mRNA?
5′ Methyl Cap
3′ Methyl Cap
Poly-A tail
5′ Methionine Cap – ANSWER- 5′ Methyl Cap
A parent has an autosomal dominant disorder. What percent chance does
this parent have to pass down this affected gene to his/her child?
0%
25%
50%
75%
100% – ANSWER- 50%
The phrase “central dogma of molecular biology” refers to the flow of
genetic data in this manner:
DNA –> RNA –> Proteins
DNA –>Proteins –> Genes
RNA –> DNA –> Proteins
RNA –> Proteins –> DNA – ANSWER- DNA –> RNA –> Proteins
What drug is NOT metabolized by CYP2D6?
Codeine
Omeprazole
Warfarin
Escitalopram – ANSWER- Warfarin
You have sequenced a gene and observe the following:
Reference: atgctggcacgacaggtttcccgactgg
Sequenced: atgCctggcacgacaggtttcccgactgg
The mutation observed is a:
Frame-shift mutation
Insertion
Silent mutation
Non-conservative mutation – ANSWER- Frame-shift mutation
A parent has an autosomal recessive disorder. What percent chance does
this parent have to pass down this affected gene to his/her child?
0%
25%
50%
75%
100% – ANSWER- 100%